Biologist - Cellular & Molecular Biology

1 day ago

Ahmedabad, India O2h Full time

Reporting to senior team leader, work with a group of in vitro assay biologists, learning and coaching group members to improve skills and productivity
- Independently design, conduct, optimize and proactively troubleshoot assays
- Develop, optimize and validate biochemical and cell-based assay platforms for the hit identification, lead optimization and functional characterization of small molecules
- Work on the bench implementing state-of-the-art technologies for the different assay platforms
- Ensure robust and high-quality assays with efficient use of automation and instrumentation
- Data processing, interpretation and presentation to cross functional team members and
- Proposing, planning, quoting, tracking reagent needs, usage and executing time lines for each study
- Work closely with other biology groups involved in screening, cell biology, protein biology and ADME, offering integrated drug discovery support alongside chemistry for multiple client-based projects

**Job requirements**:

- Experience in assay development and ability to handle 96 and 384-well formats
- Basic understanding of molecular biology, cell biology and biochemistry techniques
- Working knowledge of site-directed mutagenesis, transcriptional studies, luciferase reporter assays and qRT-PCR
- Cell culture experience and in handling different immortalized cell lines, primary cell lines and 3D cultures
- Ability to perform transient transfections and generate stable cell lines
- Good working knowledge of immunoprecipitation and Western blotting for protein analysis and quantification
- Experience in fluorescence microscopy and high-content imagining is an added advantage
- Experience in targeted protein degradation, target engagement assays (e.g. nanoBRET, CETSA) is an added advantage
- Experience in CRISPR-CAS9 knock-out and siRNa/shRNA knock-down studies is an added advantage
- Excellent data analysis and interpretation skills
- Ability to participate in project team meetings and contribute to the project(s) intellectually
- Ability to overcome technical challenges and exercise sound judgment under pressure
- High self-motivation to initiate projects and work independently under aggressive deadlines
- An excellent personal rapport and an ability to engender trust and respect
- An ability to work well as part of a small focussed team and build relationships
- An exceptional communication skill and an ease in interaction with all stakeholders
- An infectious enthusiasm for life, work and making a difference to the world in which we live
- A bright, friendly, flexible personality with agility to switch between different skill sets
- An exceptional organizational skill that thrives working in a fast-paced and vibrant team environment
- Positivity, focus and a winning mentality allied with kindness
- Curiosity, energy, creativity, and bravery with a strong desire to help to build the o2h culture

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