Assay Biologist

**Role**: Assay Biologist

**Department**:Biology

**Location**: Hyderabad

**About Us**: Sai Life Sciences is one of the fastest-growing Contract Research, Development, and Manufacturing Organizations (CRDMOs). As a pure-play, full-service partner, we collaborate with over 300 global innovator pharma and biotech companies to accelerate the discovery, development, and commercialization of their NCE programs. We have over 3000 employees across our facilities in India, the UK, and the USA, working together to deliver scientific excellence and customer success. We are a Great Place to Work certified organization with a culture of openness, mutual respect, and inclusivity. We embrace diversity and follow equal opportunity practices across all levels.

**Summary of Job**:

- Knowledge on biochemical and cell-based assays.

**Key Responsibilities**:

- Execute assay optimization, validation, and compound screening in various in vitro biochemical and cell-based assays.
- For cell-based assays hands on following is desirable:
Ø Media preparation

Ø Cell culture (Suspension and Adherent)

Ø Cell cryopreservation

Ø Cell seeding using automated dispensers

Ø Compound dilutions, manually or using Echo

Ø Hands on multimode microplate readers such as Envision and FLIPR
- For cell free (biochemical) assays understanding of following is desirable:
Ø Enzyme kinetics

Ø KM

Ø Assay optimization parameters

Ø Different assay technologies for kinase assays
- Analyse and interpret the data from assay optimization, validation, and compound screening studies.
- Record the experimental data in prescribed format in LNB and or ELN
- Operate instruments for efficient, accurate and reproducible data.
- Use analysis software/s for data handling (GraphPad Prism).
- Familiar with the current assay techniques (HTRF, AlphaLISA, Luminescene, Fluorescence, Absorbance.) and assays (Reporter gene assays, FLIPR assay, CTG assay, ADP Glo kinase assay, etc)
- Execute compound management procedures, indenting process, receiving, maintenance of reagents and consumables stocks.
- Adhere to and follow Sai policies and company rules.

**Experience/Qualification**:

- M.Sc/M.Tech/M.Pharm with 2-3 years of relevant industry experience.

**Key Competencies (Technical, Functional & Behavioural)**:
**Technical**:

- Multi-channel pipettes, Multimode plate readers, Western blot apparatus, operation of centrifuge, ELISA etc.

**Functional**:

- Basic knowledge on biochemical and cell-based assays.

**Behavioural**:

- Team player, ability to work with multidisciplinary teams.
- Strong analytical skills and ability to work under stringent timelines, good communication skills.