Cell Line development

6 days ago

tamil nadu, India Chengene Private Limited Full time

1. Role OverviewThe Scientist will be responsible for designing, executing, and optimizing processes involved in the development of mammalian cell lines and scalable upstream and downstream processes for recombinant protein production. The role involves hands-on experimental work, process characterization, and technology transfer to manufacturing.2. Key ResponsibilitiesA. Cell Line Development (CLD)Develop stable mammalian (CHO / HEK293) cell lines expressing recombinant proteins (e.g., rFSH, monoclonal antibodies, hormones, enzymes).Perform transfection, clone selection, screening, and stability studies.Utilize techniques like limiting dilution, clone picking, and high-throughput screening.Maintain detailed records of vector constructs, host cells, and clone lineage.Conduct productivity assessments (ELISA, SDS-PAGE, Western blot, HPLC).Ensure regulatory documentation for master and working cell banks (MCB/WCB).B. Upstream Process Development (USP)Design and optimize culture media, feeds, and process parameters for batch, fed-batch, and perfusion modes.Scale up processes from shake flasks → bench bioreactors → pilot scale.Perform process characterization, including DO, pH, temperature, and agitation studies.Implement Design of Experiments (DoE) for optimization.Collaborate with analytical teams for titer and quality assessments.C. Downstream Process Development (DSP)Develop purification strategies using chromatographic (Protein A, IEX, HIC, SEC) and filtration techniques (UF/DF, TFF).Optimize recovery and yield while maintaining product quality and activity.Characterize process impurities (HCP, DNA, aggregates, endotoxin).Conduct process scalability and robustness studies.Support tech transfer to GMP manufacturing.D. Cross-functional & DocumentationCollaborate with Analytical, QA, and Manufacturing teams for process integration.Maintain detailed lab notebooks, SOPs, and batch records.Present findings in technical reviews and project meetings.Adhere to biosafety and GMP/GLP guidelines.3. QualificationsEducation: M.Sc. / M.Tech / Ph.D. in Biotechnology, Biochemistry, Bioprocess Engineering, or related discipline.Experience:Scientist: 3–6 years (for Ph.D.) or 5–8 years (for M.Sc./M.Tech) in biopharmaceutical R&D.Proven experience in cell line development, upstream or downstream process development for recombinant proteins or biosimilars.4. Desired SkillsHands-on experience with CHO or HEK cell lines.Expertise in molecular cloning, transfection, and selection techniques.Familiarity with analytical tools (ELISA, HPLC, SDS-PAGE, Western blot).Knowledge of bioreactors (Applikon, Sartorius, or similar).Proficiency with chromatography systems (ÄKTA, TFF units).Understanding of QbD and regulatory expectations (ICH Q5D, Q6B).Strong analytical thinking, troubleshooting, and documentation skills.

  • Cell Line development

    5 days ago

    tamil nadu, India Chengene Private Limited Full time

    1. Role Overview The Scientist will be responsible for designing, executing, and optimizing processes involved in the development of mammalian cell lines and scalable upstream and downstream processes for recombinant protein production. The role involves hands-on experimental work, process characterization, and technology

  • Cell Line development

    6 days ago

    Chennai, Tamil Nadu, India, Tamil Nadu Chengene Private Limited Full time

    1. Role OverviewThe Scientist will be responsible for designing, executing, and optimizing processes involved in the development of mammalian cell lines and scalable upstream and downstream processes for recombinant protein production. The role involves hands-on experimental work, process characterization, and technology transfer to manufacturing.2. Key...

  • Line Qc

    2 weeks ago

    Tiruppur, Tamil Nadu, India Bose exports Full time

    **Key Responsibilities**: - **In-Line Quality Inspections**: - Conduct quality checks on garments at various stages of the production line to ensure they meet the required standards. - Inspect for defects such as incorrect stitching, fabric flaws, color discrepancies, and fitting issues. - **Documentation and Reporting**: - Maintain detailed records of...

  • Line Quality Inspector

    1 week ago

    Gummidipoondi, Chennai, Tamil Nadu, India Industrial Support services consulting Full time

    Looking Line Quality Inspector For Manufacturing Industry - **Should have experience in Quality Inspection**: - **Exp : 1 yr - 3 yrs**: - **B.E Mech / B.Tech Mech Only**: - **Food and Accommodation Provided**: - Immediate Joiner Preferred **Job Types**: Full-time, Permanent Pay: ₹18,000.00 - ₹22,000.00 per month **Benefits**: - Cell phone...

  • Cell Culture Specialist

    2 weeks ago

    tamil nadu, India Biocon Biologics Full time

    Cell culture Specialist;Skills to support Upstream process development, Process Scale up, Process characterization for mAb manufacturing.Develop and establish scalable and robust processes for meeting desired product yield and qualityAssess risk and actively plan for risk mitigation strategies during process development and scale upCollaboration with cross...

  • Line Quality

    8 hours ago

    Pammal, Chennai, Tamil Nadu, India New tech Auto Components Pvt Ltd Full time

    Roles & Responsibilities (QC) - Approve incoming materials by confirming specifications, conducting a visual and measurement test, and rejecting and returning unacceptable materials - Approve in-process production by confirming required specifications, conducting visual and measurement tests, and communicating the required adjustments to the production...

  • Marketing Executive

    6 days ago

    Coimbatore, Tamil Nadu, India Yello Line Full time

    We are LED Manufacturing Industry It's totally Marketing Related Job Qualification : Any degree Salary : 15k to 20k Openings in Coimbatore, Chennai, Vellore, Bangalore, & Hyderabad Skills and Qualifications: - Qualification any degree or MBA is added advantage. - Freshers Only - English Communication is Must - Hindi is Added advantage. - Flexibility to...

  • Marketing Executive

    1 week ago

    Coimbatore, Tamil Nadu, India Yello Line Full time

    We are LED Manufacturing Industry It's totally Field Marketing If you not willing in field marketing. this job is not suitable for you Openings in Coimbatore, Chennai, Vellore, Bangalore, & Hyderabad Skills and Qualifications: - Qualification any degree or MBA is added advantage. - Freshers Only - English Communication is Must - Hindi is Added...

  • Cell Culture Specialist

    2 weeks ago

    Chennai, Tamil Nadu, India, Tamil Nadu Biocon Biologics Full time

    Cell culture Specialist;Skills to support Upstream process development, Process Scale up, Process characterization for mAb manufacturing.Develop and establish scalable and robust processes for meeting desired product yield and qualityAssess risk and actively plan for risk mitigation strategies during process development and scale upCollaboration with cross...

  • Male Hostel Warden

    2 weeks ago

    Perungudi, Chennai, Tamil Nadu, India Life Line Placements Full time

    **Wanted Male Hostel warden for Men working Hostel Age below 45 immediately joining** **Job Types**: Full-time, Fresher, Internship **Salary**: ₹15,000.00 - ₹15,500.00 per month **Benefits**: - Cell phone reimbursement - Food provided - Leave encashment - Paid sick time Schedule: - Day shift Supplemental pay types: - Yearly...