Renowned Scientist Wanted: Advanced Molecular Biology Expert

7 days ago

Hyderabad Secunderabad Telangana, India beBeeBiologist Full time US$ 90,000 - US$ 1,20,000
Job Title:

Molecular Biologist

">Job Description:
  • Develop and execute DNA and RNA extraction protocols for various scales.
  • Design and perform PCR techniques, including inverse, touch down/up, gradient, long amplicons, TaqMan, and real-time PCR for diverse applications.
  • Optimize cloning methods via cut-and-paste, Gibson assembly, Gateway, and In-Fusion.
  • Maintain and stock cell lines, specifically HEK 293T, HeLa, MCF-7, and suspension cell lines like Expi and FreeStyle series.
  • Perform transfection using chemical and electroporation methods at varying scales.
  • Advance protein expression in bacteria, yeast, insect, and mammalian cells, and support protein separation and purification.
  • Conduct protein estimation assays (e.g., Bradford, BCA, or ELISA).
Key Responsibilities:
  • Expertly manage experiments, troubleshoot when necessary, and effectively collaborate with cross-functional teams to advance R&D projects in mRNA vaccine development, cell line generation, and protein production.
">Qualifications:
  • PhD in Molecular Biology, Cell Biology, or a related field; OR a Master's degree in a relevant field with at least 3+ years of additional experience in a relevant laboratory environment.
  • Hands-on experience with DNA and RNA extraction, as well as plasmid extraction at various scales.
  • Extensive expertise in PCR techniques, including real-time PCR and TaqMan assays, for various molecular biology applications.
  • Proficiency in cloning methods, including cut-and-paste, Gibson assembly, Gateway, and In-Fusion.
  • Experience with cell line maintenance (especially HEK 293T, HeLa, and MCF-7) and suspension cell lines like Expi and FreeStyle.
  • Expertise in transfection techniques, including chemical and electroporation methods.
  • Demonstrated ability to achieve protein expression in bacteria, yeast, insect, and mammalian cells.
  • Experience in protein separation and purification methods (e.g., FPLC).
  • Familiarity with protein estimation assays like Bradford, BCA, or ELISA, and knowledge of Western blotting for protein expression analysis.
Preferred Experience:
  • Experience working in a cGMP or regulated environment is beneficial.
  • A track record of publishing scientific findings and presenting them clearly in both written and oral formats.
What We Offer:
  • Competitive compensation package.
  • Comprehensive health insurance covering immediate family.
  • Collaborative work environment focused on innovative solutions to complex challenges in the mRNA vaccine space.
  • Career development opportunities in a rapidly expanding biotech company.
  • Chance to make a meaningful impact on global health through innovative vaccine development.
"]},

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