Stem Cell Processing Technician
4 weeks ago
Job Description: Cell Processing Technician — PRP & Bone Marrow–Derived Cell FractionsOrganisation: ALIV Regenerative WellnessLocation: Mumbai | Type: Full-time | Mode: On-siteReports to: Lab/Clinical Operations Lead1 Month TRAINING in PUNEAbout ALIVALIV is a compliant regenerative wellness clinic focused on Autologous Cell Therapy, PRP/PRFM, and targeted IV therapies, delivered with strict ethics and SOPs.Role SummaryHands-on technician to process PRP/PRFM and bone marrow–derived autologous cell fractions (minimally manipulated) with impeccable aseptic technique, documentation, and turnaround time. You’ll partner with clinicians to ensure safe, consistent, and reproducible outputs for patient procedures.Key ResponsibilitiesSample Handling & Chain of Custody- Receive, label, and track blood and bone-marrow aspirate; maintain time/temperature controls and transport logs. - Verify consent, requisitions, and kits; ensure traceability from collection to reinfusion.PRP / PRFM Processing- Perform anticoagulation, centrifugation per protocol (soft/hard spins), plasma separation, platelet resuspension, PRFM activation. - Record volumes, spin parameters, platelet yield/concentration, and lot numbers of disposables.Bone Marrow–Derived Cell Fraction Processing- Process aspirate using validated closed/open systems (e.g., RBC depletion, density separation). - Isolate mononuclear/cell fractions as per SOP; prepare final product for clinical use.Asepsis, QC & Release- Work under Class II biosafety cabinet/laminar flow; follow sterile technique and PPE. - Perform in-process QC: TNC/platelet counts, viability (e.g., Trypan Blue), recovery %, hemolysis check, appearance. - Prepare batch records and Certificate of Processing/Release for clinician sign-off.Equipment & Facility- Operate and maintain centrifuges (fixed/swing), pipettes, cell counters/hemocytometer, vortex, incubators (if applicable). - Daily/weekly calibration, IQ/OQ/PQ logs, maintenance tickets, and environmental hygiene records.Compliance & Safety- Adhere to DCGI/ICMR guidance, clinic SOPs, biosafety, biomedical waste rules, and incident/AER reporting. - Participate in audits, CAPA, and periodic validation runs.Inventory & Coordination- Manage stock of kits, anticoagulants, reagents, disposables; raise purchase requests proactively. - Coordinate OR/clinic schedules for timely processing and delivery.Qualifications- B.Sc./M.Sc. in Biotechnology/Microbiology/Biomedical/Allied Health or DMLT/BMLT. - 2–5 years in a cell/hematology/transfusion, IVF, orthobiologics, or day-care lab (PRP/BM processing preferred). - Strong grasp of aseptic technique, centrifugation theory, and basic hematology counts. - Comfortable with EMR/CRM/LIS, Google Workspace, and documentation discipline. - Languages: English + Hindi/Marathi (or local).Skills & Traits- Meticulous, steady hands, and SOP-first mindset. - Data-accurate; converts protocols into repeatable outputs. - Calm under time pressure; clear communication with clinicians and nursing. - Integrity, patient privacy, and teamwork.KPIs- Yield & viability within spec; turnaround time adherence. - Documentation accuracy (batch records, logs) and SOP compliance. - Zero contamination/incident rate; successful audits. - Stockouts avoided; equipment uptime.90-Day Outcomes- 30 days: Trained and certified on ALIV SOPs; independent PRP runs with complete records. - 60 days: Independent bone-marrow fractionation; QC dashboards live. - 90 days: ≥95% runs within yield/viability specs; audit-ready documentation; proposes at least 2 process improvements.
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