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Scientist II, Protein Biology
3 weeks ago
Job Description
About the company
Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, with annual revenue of approximately $44 billion. Our Mission is to enable our customers to make the world healthier, cleaner, and safer. Whether our customers are accelerating life sciences research, solving analytical challenges, growing efficiency in their laboratories, improving patient health through diagnostics, or developing and manufacturing life-changing therapies, we are here to support them. Our distributed team delivers an outstanding combination of innovative technologies, purchasing convenience, and pharmaceutical services through our industry-leading brands, including Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific, Unity Lab Services, Patheon, and PPD. Our Innovative Laboratory technologies & Services makes it easier for customers.
About Team: Antibody teamThe antibody team is involved in engineering and development of antibodies, with a deep focus on reagent quality and specificity. The core activities of team include design, development, purification, conjugation and testing of antibodies in a variety of immunoassay applications using pioneering tools and technologies. The goal of the program is advanced product development and creation of product specific information to promote research use.
Role & ResponsibilitiesRole:
To be an integral part of an antibody development team with a focus on performance and specificity using innovative molecular, protein, and cell biology methods.
Responsibilities:- Contribute in a fast- paced environment towards Antibody development & Validation
- Project leadership role in new antibody development programs with vast experience in experimental planning and execution to meet the program objectives.
- Lead team of scientists in matrix style operation, provide technical and operational supervision. Mentoring and training junior scientists in research,
- Design and experimental execution, interpreting data and data presentation to the stakeholders.
- Drive ideation within teams to enable development of successful protocols for effective transfer to operation teams.
- Serve as domain expert in the field of flow cytometry, cell sorting and protein biology to provide inputs and guidance towards driving innovation and cross team collaborations.
- Knowledge of product commercialization processes from concept to launch including early feasibility, development, and validation phases.
- Support manufacturing team for technical support and quality by providing technical guidance.
Education & Experience levels:
- PhD with 2 to 4 years' experience, or
- Masters degree with 5 to 10 years' experience
- Min 3-4 years of Industrial experience is required
Applicants should have a degree in a relevant life sciences field with a strong focus on sophisticated flow cytometry, cell sorting, panel design and immunology.
Nature of experience:- Strong background in flow cytometry including single cell sorting, immunophenotyping, multicolor flow cytometry and panel design, rare population analysis
- Hands-on experience with both intracellular and extracellular multi-color staining
- Understanding of cytometry data analysis techniques (e.g. population gating, compensation, etc.)
- Proficiency with flow cytometry analysis software (e.g. FlowJo, FCS Express)
- Ability to process tissue and isolate cells, particularly human/mammalian PBMCs and mouse splenocytes
- Solid understanding and hands on experience with monoclonal antibody development methods using one or more of the following technologies would be an advantage: single B cell sorting, phage display, recombinant technology
- Experience in preparing and culturing human and mouse primary immune cells
- Proficient in molecular biology workflows such as high-throughput molecular cloning, plasmid vector construction, PCRs, Restriction digestion, Ligation, Transformation, Mini and maxi preps, sequencing
- Thorough in biochemical and immunoassay workflows such as recombinant protein expression and purification, ELISA, SDS-PAGE, Western blotting, IF, IHC
- Working knowledge of fluorescence microscopy, fluorescence detection technologies is helpful
- Experience in authoring and optimizing standard operating procedures with a focus on reproducibility
- Aptitude for identifying deviations from expected outcomes, troubleshooting and suggesting alternatives
- Excellent written and verbal presentation skills
- Proven leadership skills and ability to work alongside colleagues from different backgrounds
- Ability to succeed with a moderate level of supervision and guidance
- Ability to provide technical trouble shooting to resolve scientific problems.
- Proficient in software or platforms for molecular biology and sequence analysis, MS Office and statistical software such as GraphPad Prism.
- Authorship of peer-reviewed publications with post-doctoral experience and a strong, independent understanding of standard and emerging research protocols and techniques are preferred. GLP experience is a must.
- Prior project leadership experience preferred.
The successful candidate will be self-motivated and a self-starter who is excellent at learning and applying new concepts for process or product improvements, possess excellent oral and written communication skills to connect to peers and leadership, and will be an excellent teammate with the ability to work with diverse colleagues.
Thermo Fisher Scientific is an equal opportunity employer and value diversity at our company. All qualified applicants will receive consideration for employment without discrimination basis race, color, religion, sex, sexual orientation, gender, gender identity or expression, national origin, protected veteran status, age, genetics, pregnancy, disability or any other legally protected status. Thermo Fisher Scientific aligns with all applicable equal employment opportunity legislation in each jurisdiction in which it operates.
We offer competitive remuneration, annual incentive plan bonus, healthcare, and a range of employee benefits. Thermo Fisher Scientific offers employment with an innovative, forward-thinking organization, and outstanding career and development prospects. We offer an exciting company culture that stands for integrity, intensity, involvement, and innovation
ThermoFisher is committed to crafting a diverse work environment that values and respects individuals from all backgrounds. Thermo Fisher Scientific is an EEO/Affirmative Action Employer and does not discriminate on the basis of race, color, religion, sex, sexual orientation, gender identity, national origin, protected veteran status, disability or any other legally protected status
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