UPSTREAM PROCESS
1 week ago
Role Highlights:
Execution of activities related to R&D E. coli culture and USP
Key Responsibilities:
- Cell bank preparation for E. coli vaccine candidates. Research cell bank, master cell bank working cell banks from Master Cell bank and all flask activity.
- Cultivation of various clones at shake flask level etc.
- Microbial Process development for E. coli at shake flask and 5L fermenter to achieve high cell density and expression level.
- Execution of consistency batches @ 5Litre scale and 30L Scale.
- Optimization of scale up process of final vaccine candidate from 5L scale to 20 L scale.
- Aseptic process (Media Fill) simulation studies in the R&D USP.
- Resource management for the smooth batch processing at 3rd floor USP facility.
- Documentation and report preparation for the batches. Preparation of SOP, and other QA related documents required for microbial USP facility and process.
- Literature search, paper presentation on the current technologies being used for E. coli fermentation.
- Supporting in the execution of protocols for the mammalian process optimization in wave bioreactor and stirred tank bioreactor.
- Process development report and other document preparation as per requirements.
- Technology Transfer for E.coli process @ 30 L & 50 L scale at commercial site.
- Technology transfer documentation preparation and review.
- Experience in transferring technology .from sending unit to receiving unit.
- Validation/revalidation/calibration., IQ, OQ and PQ of new equipment’s to be installed in upstream processing area.
What Makes You a Fit:
- Experience : 06 -10 years in vaccine R&D in Upstream Process
- Education : M.Sc in Biotechnology or a related field
- Skills : Microbial Fermentation & Process Development, Documentation & Compliance, Experience in scaling processes from lab to commercial scale, and technology transfer documentation)
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