Panacea Biotec | UPSTREAM PROCESS

3 weeks ago


delhi, India Panacea Biotec Full time

Role Highlights:

Execution of activities related to R&D E. coli culture and USP


Key Responsibilities:


  • Cell bank preparation for E. coli vaccine candidates. Research cell bank, master cell bank working cell banks from Master Cell bank and all flask activity.
  • Cultivation of various clones at shake flask level etc.
  • Microbial Process development for E. coli at shake flask and 5L fermenter to achieve high cell density and expression level.
  • Execution of consistency batches @ 5Litre scale and 30L Scale.
  • Optimization of scale up process of final vaccine candidate from 5L scale to 20 L scale.
  • Aseptic process (Media Fill) simulation studies in the R&D USP.
  • Resource management for the smooth batch processing at 3rd floor USP facility.
  • Documentation and report preparation for the batches. Preparation of SOP, and other QA related documents required for microbial USP facility and process.
  • Literature search, paper presentation on the current technologies being used for E. coli fermentation.
  • Supporting in the execution of protocols for the mammalian process optimization in wave bioreactor and stirred tank bioreactor.
  • Process development report and other document preparation as per requirements.
  • Technology Transfer for E.coli process @ 30 L & 50 L scale at commercial site.
  • Technology transfer documentation preparation and review.
  • Experience in transferring technology .from sending unit to receiving unit.
  • Validation/revalidation/calibration., IQ, OQ and PQ of new equipment’s to be installed in upstream processing area.


What Makes You a Fit:

  • Experience: 06 -10 years in vaccine R&D in Upstream Process
  • Education: M.Sc in Biotechnology or a related field
  • Skills: Microbial Fermentation & Process Development, Documentation & Compliance, Experience in scaling processes from lab to commercial scale, and technology transfer documentation)


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