Panacea Biotec | UPSTREAM PROCESS
3 weeks ago
Key Responsibilities:
Cell bank preparation for E. coli vaccine candidates. Research cell bank, master cell bank working cell banks from Master Cell bank and all flask activity.Cultivation of various clones at shake flask level etc.Microbial Process development for
E. coli
at shake flask and 5L fermenter to achieve high cell density and expression level.Execution of consistency batches @ 5Litre scale and 30L Scale.Optimization of scale up process of final vaccine candidate from 5L scale to 20 L scale.Aseptic process (Media Fill) simulation studies in the R&D USP.Resource management for the smooth batch processing at 3rd floor USP facility.Documentation and report preparation for the batches. Preparation of SOP, and other QA related documents required for microbial USP facility and process.Literature search, paper presentation on the current technologies being used for E. coli fermentation.Supporting in the execution of protocols for the mammalian process optimization in wave bioreactor and stirred tank bioreactor.Process development report and other document preparation as per requirements.Technology Transfer for
E.coli
process @ 30 L & 50 L scale at commercial site.Technology transfer documentation preparation and review.Experience in transferring technology .from sending unit to receiving unit.Validation/revalidation/calibration., IQ, OQ and PQ of new equipment’s to be installed in upstream processing area.
What Makes You a Fit:Experience : 06 -10 years in vaccine R&D in Upstream ProcessEducation : M.Sc in Biotechnology or a related fieldSkills : Microbial Fermentation & Process Development, Documentation & Compliance, Experience in scaling processes from lab to commercial scale, and technology transfer documentation)
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