▷ [High Salary] UPSTREAM PROCESS - R&D
3 weeks ago
Execution of activities related to R&D E. coli culture and USP
Key Responsibilities:
- Cell bank preparation for E. coli vaccine candidates. Research cell bank, master cell bank working cell banks from Master Cell bank and all flask activity.- Cultivation of various clones at shake flask level etc.- Microbial Process development for E. coli at shake flask and 5L fermenter to achieve high cell density and expression level.- Execution of consistency batches @ 5Litre scale and 30L Scale.- Optimization of scale up process of final vaccine candidate from 5L scale to 20 L scale.- Aseptic process (Media Fill) simulation studies in the R&D USP.- Resource management for the smooth batch processing at 3rd floor USP facility.- Documentation and report preparation for the batches. Preparation of SOP, and other QA related documents required for microbial USP facility and process.- Literature search, paper presentation on the current technologies being used for E. coli fermentation.- Supporting in the execution of protocols for the mammalian process optimization in wave bioreactor and stirred tank bioreactor.- Process development report and other document preparation as per requirements.- Technology Transfer for E.coli process @ 30 L & 50 L scale at commercial site.- Technology transfer documentation preparation and review.- Experience in transferring technology .from sending unit to receiving unit.- Validation/revalidation/calibration., IQ, OQ and PQ of new equipment’s to be installed in upstream processing area.
What Makes You a Fit:
- Experience: 06 -10 years in vaccine R&D in Upstream Process- Education: M.Sc in Biotechnology or a related field- Skills: Microbial Fermentation & Process Development, Documentation & Compliance, Experience in scaling processes from lab to commercial scale, and technology transfer documentation)
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